|
KMID : 0624620100430010057
|
|
BMB Reports
2010 Volume.43 No. 1 p.57 ~ p.61
|
|
|
Over-expression of JunB inhibits mitochondrial stress and cytotoxicity in human lymphoma cells exposed to chronic oxidative stress
|
|
Son Young-Ok
Kim Jong-Ghee
Jeon Young-Mi
Lee Jeong-Chae
Kim Tae-Geum
Heo Jung-Sun
|
|
|
Abstract
|
|
|
|
Activator protein-1 can induce either cell survival or death, which is controlled by opposing effects of different Jun members. It is generally accepted that c-Jun is pro-apoptotic, but that JunD is anti-apoptotic in stress-exposed cells. Additionally, although there are reports suggesting that JunB plays a protective role, its role in stress-induced apoptosis remains unclear. Here, we investigated the role of JunB in H2O2-induced cell death using cells that over-expressed the protein or were transfected with si-JunB. Inhibition of JunB expression accelerated H2O2-mediated loss of mitochondrial membrane potential (MMP) and cytotoxicity. Conversely, over-expression of JunB protein led to significant inhibition of the MMP loss and cell death. The increase in JunB expression also attenuated nuclear relocation of apoptosis-inducing factor and mitochondrial Bcl-2 reduction that occurred following H2O2 exposure. These results suggest that JunB can signal survival against oxidant-mediated cell death by suppressing mitochondrial stress.
|
|
|
KEYWORD
|
|
|
Human lymphoma cells, Hydrogen peroxide, JunB, Mitochondrial stress, Oxidative stress
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|